| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cullen, Casey S (12)
|
MAPWAC |
28.84 |
28.53 |
-0.31 |
| 2 |
Warner, Lyndsey J (12)
|
MAGPAC |
28.82 |
29.26 |
0.44 |
| 3 |
Thompson, Paris J (12)
|
MASAKA |
29.48 |
30.14 |
0.66 |
| 4 |
Conti, Amanda H (11)
|
MAPWAC |
32.00 |
31.87 |
-0.13 |
| 5 |
Okongwu, Ijenu U (12)
|
MASERP |
38.11 |
32.27 |
-5.84 |
| 6 |
Teofanova, Lilly A (12)
|
MAPVA |
33.99 |
33.18 |
-0.81 |
| 7 |
Mathieu, Talicia S (11)
|
MAPVA |
34.67 |
34.15 |
-0.52 |
| 8 |
McElwain, Audrey S (12)
|
MAJRAY |
34.53 |
35.52 |
0.99 |
| 9 |
Gray, Elizabeth C (11)
|
MASERP |
36.90 |
36.64 |
-0.26 |
| 10 |
Danastasio, Giovanna M (11)
|
MAWAVE |
38.04 |
36.93 |
-1.11 |
| 11 |
Montgomery, Kayleigh M (11)
|
MAPVA |
38.69 |
39.25 |
0.56 |
|
Stuart, Natalie M (12)
|
MAPWAC |
31.90 |
NS |
- |
|
Henry, Kameryn L (12)
|
MASERP |
41.99 |
NS |
- |
|
Schwab, Mary Ruth M (11)
|
MASERP |
|
NS |
- |
|
Wilson, Mekhi S (12)
|
MASSC |
31.00 |
NS |
- |